【Product Name】Hawthorn Leaf Extract

【Plant Source】Dried Leaf of Crataegus pinnatifida Bge.var. major N.E.Br. or Dried Leaf of Crataegus pinnatifida Bge. 

【Method】 

Take hawthorn leaves, crushed into coarse powder, add 50% ethanol extracted twice (55-60℃), every 2 hours, adding 10 times the first, second 8 times the amount of filtration, combined filtrate, recovery of ethanol to the filtrate no alcohol flavor, diluted with an equal amount of water by D101 macroporous resin column, followed by water and different concentrations of ethanol, the corresponding eluate collection, recycling ethanol, concentrated to the relative density of about 1.10 (60 ℃) the clear cream, spray drying, that is, too. 

【Properties】 This product is light brown to yellow brown powder; gas specific, bitter, has cited moist. 

【Identification of this product】 

5mg, 2ml dissolved with methanol, filtration, the filtrate as the test solution. In another Vitexin rhamnoside reference substance, add methanol produced per 1ml of solution containing 1 mg, as the reference solution. According to thin layer chromatography (Appendix Ⅵ B) test, to learn the two solutions of the 2-3  l, respectively, points on the same silica gel GF254 thin-layer plate with ethyl acetate - methanol - water (25:5:3) as the agent, started out, drying, home UV light (254nm), under review. Test products for chromatography, chromatography with reference substance corresponding position, was the same color of the fluorescent spots. 

【loss on drying of this product Check】 

1g, accurately weighed, dried to constant weight set weighing bottle, in sulfuric acid desiccator dry for 24 hours, not less than 2.0% weight loss (Appendix IX G). 

【Characteristic】 

spectrum for high performance liquid chromatography (Appendix VI D) determined. 

【Chromatographic conditions and system suitability test】 

Octadecyl silane bonded silica as a filler; to tetrahydrofuran - methanol - acetonitrile - acetic acid - water (38:3:3:4:152) as mobile phase; the detection wavelength was 330nm. Number of theoretical plates vitexin rhamnoside peak should not be less than 2500. 

【Preparation of reference solution】 vitexin rhamnoside take appropriate reference substance, accurately weighed, add 60% ethanol produced per 1ml of solution containing 100ug, that is, too. 

【Preparation of the test solution】 to take this product 50mg, accurately weighed, home 50ml volumetric flask, add 60% ethanol, dissolved and diluted to the scale, that is, too. 

【Tested method】Determination of reference were drawn precision solution and sample solution of the 10ul, into the liquid chromatograph to measure and record the chromatogram, that is, too. 

Characteristics of the test pattern should be presented in four characteristic peaks with reference peaks corresponding peak S peak, calculate the characteristic peaks of the relative retention time S should be specified within ± 5%. Provisions of the relative retention time values are: 0.76 (peak 1), 1.00 (peak S), 1.55 (peak 2), 1.94 (peak 3). 

Peak 1: Vitexin glucosaminidase;   Peak S: vitexin rhamnoside;   

Peak 2: Vitexin                               Peak 3: Hyperoside

Integral parameters of the slope sensitivity of 5, the peak width of 0.04, the minimum peak area of 10, the minimum peak height of peak for the S 1% higher. 

【Assay Preparation of control solution flavonoids rutin take appropriate reference substance, accurately weighed, add ethanol made from 0.20mg per 1ml of solution containing rutin (ultrasonic treatment allows the dissolved when necessary), that is, too. 

Preparation of standard curve precise amount of the reference solution 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 25ml volumetric flasks were set, and add water to 6ml, plus 5% sodium nitrite solution 1ml, to mix, place 6 minutes , plus 10% aluminum nitrate solution 1ml, shake, for 6 minutes, add sodium hydroxide test solution 10ml, add water to the mark, shake, for 15 minutes, to the corresponding reagent blank, according to ultraviolet - visible spectrophotometry (Appendix Ⅴ A), was measured by absorbance wavelength of 500nm to absorbance as the ordinate, concentration as abscissa, the standard curve. 

Determination of this product 0.15g, accurately weighed conical flask with stopper set, precision dilute ethanol 25ml, Mesa, shake, ultrasonic treatment for 5 minutes for 3 hours, filtration, precise amount of filtrate added 2ml, home 25ml volumetric flask, diluted with water to the mark, shake, as the test solution. Precise amount of the test solution 2ml, 25ml volumetric flask set, according to the standard curve method under preparation, since the "add water to the 6ml" since, according to the absorbance measurement, and precise amount of the test solution 2ml, 25ml set volumetric flask, add water to the mark, shake, as the blank solution. Read from the standard curve for rutin in the test solution, calculation, that is, too. 

This product calculated on dry goods, including rutin flavonoids anhydrous (C 27 H 30 O 16) dollars, not less than 80.0%. 

Vitexin rhamnoside for high performance liquid chromatography (Appendix Ⅵ D) determined. Chromatographic conditions and system suitability test with the [feature map] under / 

Preparation of control solution vitexin rhamnoside take appropriate reference substance, accurately weighed, add 60% ethanol produced per 1ml of solution containing 100ug, that is, too. 

Preparation of the test solution to take this product 50mg, accurately weighed, home 50ml volumetric flask, add 60% ethanol, dissolved and diluted to the scale, that is, too. 

Determination of precision drawing reference solution, respectively, the test solution with the 10ul, into the liquid chromatograph to measure, that is, too. 

This product calculated on dry goods, including vitexin rhamnoside (C 27 H 30 O 14) not less than 8.8%. 

【Storage】 Sealed, cool and dry place.

Wincobel is the main manufacturer of Hawthorn Leaf Extract and Hawthorn Berry Extract in China, thanks for sending inquiry.